Superoxide production by stimulated equine polymorphonuclear leukocytes-inhibition by anti-inflammatory drugs     

D. E. AUER  J. C. NG  A. A. SEAWRIGHT 《Journal of veterinary pharmacology and therapeutics》1990,13(1):59-66

Polymorphonuclear leukocytes (PMNLs) were isolated from an inflammatory exudate induced in the intercarpal joints of horses by an administration of carrageenin. Their superoxide production at rest and following stimulation with either serum-treated zymosan (STZ) or phorbol myristate acetate (PMA) was measured by cytochrome-c reduction. Stimulation of the cells increased the cytochrome-c reduction 10-15 times that of resting cells. The maxima were 20 nmol of reduced cytochrome-c per 10(6) cells per ml at 120 min (STZ) and 35 nmol of reduced cytochrome-c per 10(6) cells per ml at 60 min (PMA). The maximum inhibition of the cytochrome-c reduction by superoxide dismutase (Palosein) was 83.6% (STZ stimulation) and 72.1% (PMA stimulation). The non-steroidal anti-inflammatory drugs, phenylbutazone, salicylic acid, aspirin, sodium salicylate in addition to D-penicillamine and dimethylsulfoxide caused dose-dependent inhibition of the cytochrome-c reduction when the cells were stimulated by PMA. The maximum inhibitions were 64% and 36% for aspirin (10(-2) M), 32% and 17% for phenylbutazone (10(-3) M), 15% and 31% for dimethylsulfoxide (6.4 x 10(-1) M), 32% and 19% for salicylic acid (10(-2) M), 0% and 17% for sodium salicylate (10(-2) M) and 2.2% and 2.5% for D-penicillamine (10(-4) M) when the cells were stimulated by STZ and PMA, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   

百合无病毒苗快速繁殖技术   总被引：3，自引：0，他引：3  

张建华  庄天明  陈银华 《上海交通大学学报(农业科学版)》2006,24(4):370-373

以东方百合珠芽作为外植体进行脱毒苗的组织培养快速繁殖技术的研究,结果表明诱导愈伤组织的最适培养基为MS附加BA0.5mg·L-1和NAA0.1mg·L-1,培养7d后愈伤组织就启动,愈伤组织的诱导率达到90.5%;最适宜分化的培养基为MS BA1.5mg·L-1 KT0.1mg·L-1 NAA0.1mg·L-1,培养8d即开始分化出丛状芽,分化率可达到93.33%;最适宜芽增殖培养基为MS附加BA0.5mg·L-1和NAA0.05mg·L-1,繁殖系数达到5.4;无根苗转入1/2MS IAA1.0mg·L-1,100%生根;经检测,0.3～0.8mm的外植体,均能达到脱毒的要求。  相似文献   

瘤胃稳定性脂肪对产后奶牛奶产量、牛奶成份和外周血白细胞数量的影响     

张克春  谭勋  王小龙 《上海交通大学学报(农业科学版)》2006,24(4):345-348

选择经产、胎次相同和产奶量相近的荷斯坦奶牛40头,配对分组设计分为对照组和试验组,每组20头。对照组全程饲喂基础日粮,试验组饲喂基础日粮 瘤胃稳定性脂肪。结果:瘤胃稳定性脂肪对奶牛采食量无显著影响(P>0.05);试验组牛产奶量显著升高(P<0.05),乳脂率呈上升趋势(P>0.05),但乳蛋白率未见显著变化(P>0.05);试验组奶牛外周血白细胞总数和淋巴细胞数轻度上升(P>0.05),牛奶SCC呈下降趋势(P>0.05)。结果表明,补充瘤胃稳定性脂肪可提高产奶量,对于改善机体免疫机能有一定的作用。  相似文献   

‘桂热杧82号’花期短截花枝试验     

陈千付  韦淑丹  钟勇  黄杰  王烨熔  黄慧俐  杨谨瑛  蒋强  曹元鑫 《中国热带农业》2021,(2):61-64,48

为延迟‘桂热杧82号’采收期,提高杧果种植效益,对‘桂热杧82号’在不同花期进行短截花枝处理试验。结果表明,在原生花序初花期、盛花期短截花枝并配套相应的栽培技术,‘桂热杧82号’均易抽出再生花序,再生花序盛花期较对照分别推迟约30~35d、40~50d,采收期较对照分别推迟约30d、40d,且产量较对照显著增加,分别是对照的1.63倍、2.25倍;有胚果数量较对照明显增多,分别是对照的1.39倍、2.25倍;采收期果实品质与对照无明显差异。原生花序初花期、盛花期短截花枝是推迟‘桂热杧82号’上市的有效措施。  相似文献   

基于PCA的新疆生产建设兵团城镇化评价指标体系的构建     

郭丽峰  张吉林  陈小丽 《安徽农业科学》2016,(28)

结合新疆生产建设兵团城镇化建设自身特点,将生态环境指标和社会稳定指标作为重要的考核因素,形成一种全方位的城镇化综合评价因子。以《新疆生产建设兵团统计年鉴2014》中兵团14个师的数据为例,运用PCA方法,将影响评价兵团城镇化发展水平的20个主要因子,分为社会发展、经济建设、基础建设、社会稳定和生态环境5个方面,构建了兵团城镇化发展的新型评价指标体系。  相似文献   

Landscape dynamics in endangered cork oak woodlands in Southwestern Portugal (1958–2005)     

Augusta Costa  Helena Pereira  Manuel Madeira 《Agroforestry Systems》2009,77(2):83-96

Cork oak landscape dynamics were assessed over a 47-year period (1958–2005) in an endangered region of southwest Portugal using a GIS approach. The area of cork oak woodlands was maintained during this period, but shifts due to land abandonment were evident leading to transformation of cork oak agriculture areas into woodlands and of cork oak woodlands into shrublands (average yearly change rates of 0.6 and 0.1%, respectively). The multi-temporal landscape analyses showed that expansion and regression rates of cork oak forests were similar (15 and 16 ha year⁻¹, respectively). The main factor determining oak woodlands expansion and regression was related to land-use changes, but slope, aspect and soil type were also significant factors. The substitution of agriculture lands and oak woodlands by shrublands has a determining role in periods of disturbance and recovery of these Mediterranean ecosystems.  相似文献   

60Co-γ射线辐射处理陆地棉后代性状变异分析     

邵琳  陈勋基  王浩  危晓薇  李建平  黄全生 《新疆农业科学》2010,47(12):2350

[目的]研究不同诱变剂量对棉花遗传变异的影响.[方法]利用100～300Gy的60Co-γ射线对5个陆地棉材料干种子进行辐射处理,对其M3、M4农艺经济性状的遗传变异进行分析.[结果]5个品种(系)在200Gy诱变剂量达到半致死剂量,200～300Gy是陆地棉理想的诱变剂量.M4群体的单铃重、单株铃数、株高和果枝数平均变异系数均超过10;以上,250Gy剂量诱变的辐射诱变后代M4群体表型性状变异系数存在明显差异.[结论]200～300Gy辐射剂量对不同棉花品种的诱变效果存在差异,揭示了辐射创造丰富的遗传变异.  相似文献   

Analysis of spatial patterns of oak decline in cork oak woodlands in Mediterranean conditions     

Augusta Costa  Helena Pereira  Manuel Madeira 《Annals of Forest Science》2010,67(2):204-204

? Cork oak mortality is a recurrent problem in southwestern Portugal. Despite the perception of increasingly visible damage in oak woodlands on drought-prone sites, the role of the various environmental factors in their decline is not clear.

? To describe the spatial patterns of cork oak (Quercus suber L.) mortality, a cork oak mortality index (MI) was determined for each landscape feature (agroforestry system, soil type, slope and aspect) using a GIS approach. To achieve this goal, a logistic regression model was formulated analyzing interactions between landscape attributes and allowing a prediction of cork oak mortality.

? Maximum values of MI were found in (i) shrublands and open woodlands with shrub encroachment (MI 6 and 3, respectively), where competition for soil water between tree and understory increases; and (ii) on lower slopes in the rounded hilltops and smooth hillsides or shallow soils where access to groundwater resources during summer drought is difficult.

? The model highlighted the importance of the agroforestry systems on cork oak mortality and may be used to identify sensitive areas where mitigation actions should be employed in a scenario of increasing drought severity in these Mediterranean ecosystems.

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Influence of Quercus ilex trees on herbaceous production and nutrient concentrations in southern PortugalElena Cubera1, 2     

Jorge Manuel Nunes  Manuel Madeira  Luíz Gazarini 《植物养料与土壤学杂志》2009,172(4):565-571

In an open woodland in Portugal, the nature of interactions between Quercus ilex trees and herbaceous plants was assessed during 2 years by studying how manipulation of incident solar radiation, water and nutrient supply affect the herbaceous biomass and N, K, P, Ca, Mg, and Mn concentrations. Measurements were carried out in three environments consisting of (1) open grassland, (2) beneath the tree canopy, and (3) under artificial shade. Each of these environments was subjected to two regimes of fertilization and two water levels in a factorial design. The fertilizer treatment consisted of application of no fertilizer or a combination of 200 kg calcium ammonium nitrate ha^–1 (26% N) and 350 kg superphosphate ha^–1 (8% P), while the water‐supply treatment consisted of either no irrigation or irrigation fortnightly from February 1 to April 30. Grasses showed significantly lower nutrient concentrations than forbs. However, nutrient concentrations of the whole herbaceous community were within the recommended ranges for cattle nutrition. A negative effect of shade on herbaceous biomass production was observed. The effect of watering on herbaceous biomass was less prominent than the effect of fertilization, irrespective of the environment, suggesting that Q. ilex does not compete for soil‐water resources with herbaceous biomass in this ecosystem. Fertilization increased total biomass by 106%, 49%, and 97% in the open grassland, beneath the tree canopy, and under artificial shade, respectively. During the first and second year, fertilization increased herbaceous P concentrations by 24% and 83%, respectively, if compared with concentrations obtained at the unfertilized plots. Higher K and Mg concentrations were observed in herbaceous plants beneath the tree canopy than in the open areas, indicating a positive effect of trees on pasture quality. The positive and negative effects of trees on understory forage are discussed.  相似文献   

Effect of bentonite characteristics on the elemental composition of wine   总被引：3，自引：0，他引：3  

Catarino S  Madeira M  Monteiro F  Rocha F  Curvelo-Garcia AS  de Sousa RB 《Journal of agricultural and food chemistry》2008,56(1):158-165

Physical, chemical, and mineralogical characteristics of six bentonites were assessed and related to their elemental release to wine. Extraction essays of bentonites in wine at three pH levels were carried out. The multielemental analysis of bentonites and wines was performed by atomic absorption spectrometry (AAS) and inductively coupled plasma mass spectrometry (ICP-MS). Bentonite addition resulted in significantly higher concentrations of Li, Be, Na, Mg, Al, Ca, Sc, V, Mn, Fe, Co, Ni, Ga, Ge, As, Sr, Y, Zr, Nb, Mo, Cd, Sn, Sb, Ba, W, Tl, Bi, and W. In contrast, the concentrations of B, K, Cu, Zn, and Rb significantly decreased. A strong correlation between Na concentrations of treated wines and its content in bentonite exchange complex was observed. Al and Fe contents reflected bentonite extractable aluminous and ferruginous constituents, while Be, Mg, Ca, V, Mn, Ni, Ge, Zr, Nb, Mo, Sn, Sb, Tl, Pb, and U concentrations reflected the elemental composition of bentonites. Several nonconformances with OIV specifications demonstrated the need for an effective control.  相似文献